Epstein-Barr Virus LMP1 Modulates the CD63 Interactome

Tetraspanin CD63 is a cluster of cell surface proteins with four transmembrane domains; it associated tetraspanin-enriched microdomains and typically localizes to late endosomes lysosomes. plays an important role in the cellular trafficking different proteins, EV cargo sorting, vesicle formation. We have previously shown that LMP1 EVs, this also affects LMP1-mediated intracellular signaling including MAPK/ERK, NF-?B, mTOR activation. Using BioID method combined mass spectrometry, we sought define broad interactome how modulates network interacting proteins. identified total 1600 as proximal CD63. Biological process enrichment analysis revealed significant involvement signal transduction, communication, protein metabolism, transportation. The CD63-only was enriched Rab GTPases, SNARE sorting nexins, while adding into increased presence ribosomal Our results showed alters interactome, shifting from localization vesicle-mediated transportation metabolic processes translation. show interacts mTOR, Nedd4 L, PP2A, indicating formation multiprotein complex CD63, thereby potentially regulating LMP1-dependent signaling. Collectively, comprehensive provides insights partners required for endocytic extracellular formation, secretion.